Composite

Part:BBa_K3105685:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-12)


Expression construct AAO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1356
    Illegal BamHI site found at 1543
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2503
    Illegal AgeI site found at 3116
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part was codon optimised for Pichia pastoris

Source

AAO basic part from Pleurotus eryngii

References